Welcome to the world of shimeji mushroom cultivation! Dive into the fascinating realm of growing your own nutrient-rich fungi with our expert guidance. Whether you're a seasoned mushroom enthusiast or a curious beginner, our comprehensive resources will walk you through the process of creating the perfect environment for your shimeji mushrooms to thrive. From selecting the right growing medium to maintaining optimal conditions, we've got you covered every step of the way. Join us on this exciting journey and unlock the health benefits and culinary delights of homegrown shimeji mushrooms.
Natural Habitat
• Ecosystem:
Cultivated
• Locations:
Worldwide
• Seasons:
Summer to Fall
• Climate:
Temperate
• Substrate:
Wood, grain
Growing Params
• Difficulty:
Moderate
• Total Time:
29 - 48 Days
• PH Range:
6 - 7
Yield Performance
• Units:
% Weight of Substrate
• Poor:
10
• Average:
20
• Good:
30
• Excellent:
40
Culture Instructions
Agar Cultivation
Supplies Needed
- • Agar Powder: Cellular medium for agar plates.
- • Petri Dishes: Containers for pouring agar medium.
- • Agar Jar: Narrow Mouth Jar with Sealing Lid is optimal.
- • Parafilm: For Sealing the agar after innoculation. Grafting Tape is also sufficient.
- • Scissors: Tool for cutting sealing tape to length.
- • Stir Plate: Optional: Thorough mixing with sagenetic stirrir improves results by distributing nutrients evenly
- • Nutrient Medium: Nutrition can be Light Malt Extract, Honey, Karo ext. For Mycelial Expansion.
- • Suppliments: Suppliments not required, but can sometimes be added to the liquid culture medium to increase growth rates.
- • Clean Environment: Flow Hood is optimal, but a still air box can be used.
- • Pressure Cooker: Equipment for sterilizing agar mixture and Petri dishes.
- • Scalpel or Innoculation Loop: Tool for transferring mycelium to agar plates.
- • Alcohol Lamp or Burner: Sterilization tool for flame sterilizing instruments.
Growing Params
Recipe: MEA
Temperature Range:70 - 75 °F
CO2 Range:5000 - 10000 ppm
Growth Duration:4 - 8 Days
Cultivation Notes: Standard agar conditions
Liquid Culture
Supplies Needed
- • Sterile Jar: Clean container for liquid culture inoculation.
- • Modified Lid: Lid should have a filter port for gas exchange. Syringe Port Optional.
- • Clean Environment: Flow Hood is optimal, but a still air box can be used.
- • Nutrient Medium: Nutrition can be Light Malt Extract, Honey, Karo ext. For Mycelial Expansion.
- • Suppliments: Suppliments not required, but can sometimes be added to the liquid culture medium to increase growth rates.
- • Pressure Cooker: Equipment for sterilizing liquid culture medium.
- • Scalpel or Syringe: Tool for transferring mycelial mass to growth medium.
- • Alcohol Lamp or Burner: Sterilization tool for flame sterilizing instruments.
Growing Params
Recipe: MDPLC
Temperature Range:70 - 75 °F
CO2 Range:5000 - 10000 ppm
Growth Duration:8 - 14 Days
Cultivation Notes: Standard LC conditionsStir Daily
Agar Cultivation
- Prepare the agar medium by dissolving agar powder in water and heating it until it boils.
- Pour the agar medium into petri dishes and allow it to solidify.
- Sterilize the petri dishes in an autoclave or pressure cooker to kill any contaminants.
- Inoculate the agar medium with shimeji mushroom spores or mycelium culture using a sterile technique.
- Seal the petri dishes with parafilm or tape to prevent contamination.
- Incubate the inoculated petri dishes in a dark, warm environment to allow the mycelium to grow and colonize the agar medium.
- Check the petri dishes regularly for contamination or mold growth, and discard any contaminated dishes.
- Once the mycelium has fully colonized the agar medium, you can use it to inoculate substrate for fruiting the shimeji mushrooms.
Liquid Culture Cultivation
- Prepare the liquid culture medium by combining water, malt extract, and agar in a sterilized container.
- Mix the ingredients thoroughly and sterilize the medium using a pressure cooker or autoclave.
- Allow the medium to cool to room temperature before inoculating it with shimeji mushroom spores or mycelium.
- Use a sterile syringe to transfer a small amount of spores or mycelium into the liquid culture medium.
- Seal the container with a breathable lid or cover to allow for gas exchange.
- Incubate the liquid culture at the optimal temperature for shimeji mushroom growth, typically around 75-80°F (24-27°C).
- Monitor the liquid culture for signs of growth, such as cloudiness or mycelium growth.
- Once the liquid culture is fully colonized with mycelium, it can be used to inoculate substrate for mushroom cultivation.
Spawn Instructions
Supplies Needed
- • Mason Jars or Bags: Containers for sterilizing and inoculating grains.
- • Modified Lid: Lid should have a filter port for gas exchange. Syringe Port Optional.
- • Grains: Substrate material for spawn inoculation. (e.g. Rye, Millet)
- • Clean Environment: Flow Hood is optimal, but a still air box can be used.
- • Suppliments: Suppliments not required, but can sometimes be added to the grain medium to increase growth rates.
- • Pressure Cooker: Equipment for sterilizing grain substrate.
- • Inoculation Tool : Tool for transferring colonized spawn to substrate. (e.g. Scalpel, Syringe)
- • Alcohol Lamp or Burner: Sterilization tool for flame sterilizing instruments.
Growing Params
Spawn Material: Whole Oats
Temperature Range:70 - 75 °F
Moisture Content:49 - 55 %
Fresh Air Exchange:0 - 1 / Hour
CO2 Range:5000 - 10000 ppm
Growth Duration:10 - 14 Days
Grain Spawn Prep and Innoculation
- Prepare your grain spawn by mixing together 2 parts water and 1 part grain (such as rye, millet, or wheat) in a large pot.
- Bring the mixture to a boil, then reduce heat and simmer for about 30 minutes or until the grains are fully cooked and have absorbed most of the water.
- Once the grains have cooked, drain any excess water and allow them to cool to room temperature.
- Transfer the cooled grains to a clean, sterile container such as a glass jar or plastic bag. Make sure the container has a breathable lid or filter to allow for gas exchange.
- Inoculate the grain spawn with your shimeji mushroom culture. You can do this by adding a small amount of the culture to the grains and mixing thoroughly.
- Seal the container with the inoculated grain spawn and incubate it in a warm, dark place for about 2-3 weeks or until the grains are fully colonized with mycelium.
- Once the grain spawn is fully colonized, it is ready to be used to inoculate your substrate for growing shimeji mushrooms. Follow the instructions for substrate preparation and inoculation to continue the growing process.
Substrate Instructions
Supplies Needed
- • Substrate Mix: Substrate material for mushroom growth. (e.g., Sawdust, Wood Chips, Fast Fruiting Mix, Brown Rice)
- • Suppliments: Suppliments not required, but can sometimes be added to the grain medium to increase growth rates.
- • Substrate Container: Polypropylene Bags or Plastic Containers for sterilizing and inoculating substrate.
- • Mycelial Mass: Typically Colonized Grain Jars or Liquid Culture for inoculation.
- • Clean Environment: Flow Hood is optimal, but a still air box can be used.
- • Pressure Cooker or Steam Sterilizer: Equipment for sterilizing substrate.
Growing Params
Substrate: Hardwood Sawdust
Sub to Spawn Ratio: 3:1
Sub to LC Ratio: 4ml/qt
Temperature Range:70 - 75 °F
Humidity Range:90 - 100 RH
Fresh Air Exchange:1 - 0 / Hour
CO2 Range:5000 - 10000 ppm
Growth Duration:10 - 14 Days
Substrate Prep and Innoculation
- Prepare the substrate by mixing hardwood sawdust and wheat bran in a ratio of 5:1.
- Moisten the substrate mixture until it reaches a field capacity moisture level.
- Fill sterilized bags with the substrate mixture, leaving some room at the top for expansion.
- Sterilize the filled bags in a pressure cooker at 15 psi for 2 hours to kill any competing organisms.
- Allow the bags to cool down to room temperature in a clean environment.
- Inoculate the cooled bags with shimeji mushroom spawn by injecting it into the substrate using a sterile syringe.
- Seal the bags with a breathable filter patch to allow for gas exchange.
- Incubate the inoculated bags in a dark, warm (75-80°F) environment for 2-3 weeks, checking for signs of colonization.
- Once the substrate is fully colonized with mycelium, move the bags to a cooler (60-65°F), well-lit area to induce fruiting.
- Maintain humidity levels around 90% and mist the bags regularly to promote mushroom growth.
- Harvest the shimeji mushrooms when they reach the desired size by twisting them off the substrate.
Primordia Instructions
Supplies Needed
- • Fruiting Chamber: Controlled environment chamber for pinning and fruiting.
- • Control Automation: Optional. Controller and sensors for maintaining temperature, humidity, lighting ext.
- • Casing Layer: Optional. Species Dependant. Humidification material for maintaining humidity in the fruiting chamber.
- • Hygrometer: Instrument for measuring humidity levels.
- • Humidifier: Optional. Could be spray bottle or humidifer. Tool for misting and humidifying the fruiting chamber.
Growing Params
Casing Layer: None
Temperature Range:50 - 60 °F
Humidity Range:90 - 100 RH
Light Wave Length:395 - 480 nm
Light Intensity:500 - 600 Lux
Light Duration: 12 Hours/Day
Fresh Air Exchange:4 - 8 / Hour
CO2 Range:500 - 1000 ppm
Growth Duration:7 - 12 Days
Cultivation Notes: Initial pinning phase
Primordia Initiation
- Prepare a clean and sanitized growing area with controlled temperature and humidity levels.
- Mix the substrate ingredients thoroughly, ensuring proper moisture content and pH levels.
- Fill the growing containers with the substrate mixture, leaving some space at the top for the mushrooms to grow.
- Inoculate the substrate with shimeji mushroom spawn, distributing it evenly throughout the container.
- Cover the containers with a breathable lid or plastic wrap to maintain humidity levels and prevent contamination.
- Place the containers in a dark, warm location (around 75-80°F) to encourage primordia formation.
- Monitor the containers daily for signs of primordia initiation, such as small pinheads forming on the surface of the substrate.
- Once primordia have formed, adjust the temperature and humidity levels slightly to promote healthy mushroom growth.
- Continue to monitor and care for the mushrooms as they mature, harvesting them at the optimal stage for consumption.
Fruiting Instructions
Supplies Needed
- • Fruiting Chamber: Controlled environment chamber for fruiting.
- • Temperature Control System: Equipment for maintaining optimal fruiting temperatures.
- • Fresh Air Exchange System: System to provide adequate fresh air exchange in the fruiting chamber.
- • Light Source: Light source to provide required illumination for fruiting. (e.g., LED Grow Light)
- • Harvesting Tools: Tools for harvesting mushrooms without damaging the mycelium. (e.g., Knife, Scissors)
Growing Params
Temperature Range:55 - 65 °F
Humidity Range:90 - 95 RH
Light Wave Length:395 - 480 nm
Light Intensity:400 - 600 lux
Light Duration: 12 Hours/Day
Fresh Air Exchange:2 - 4 / Hour
CO2 Range:2000 - 4000 ppm
Growth Duration:5 - 10 Days
Flushes:1 - 2
Flushing Schedule:21 - 25 Days
Cultivation Notes: Standard fruiting conditions
Fruiting Preparation and Conditions
- Prepare a fruiting chamber by filling it with a layer of damp perlite or vermiculite to maintain humidity.
- Place a tray or container filled with pasteurized substrate (such as sawdust or straw) in the fruiting chamber.
- Cover the substrate with a layer of casing material, such as peat moss or coconut coir, to provide a microclimate for the mushrooms to grow.
- Mist the casing layer with water to keep it moist, but be careful not to oversaturate it.
- Maintain a temperature of around 75-80°F (24-27°C) and high humidity levels (around 90%) in the fruiting chamber.
- Provide indirect light or low-intensity fluorescent light to simulate natural daylight for 12 hours a day.
- Monitor the humidity levels and mist the casing layer as needed to prevent it from drying out.
- After about 7-10 days, small mushroom pins should start to form. Continue to mist and maintain the conditions until the mushrooms are ready to harvest.