Golden Oyster Culture Slant

Product Overview

The Golden Oyster Culture Slant is a premium, sterile culture of the Pleurotus citrinopileatus species, celebrated for its stunning golden-yellow caps and rapid growth. This culture slant is perfect for mycology enthusiasts, researchers, and educators, providing a stable and reliable starting point for propagation, research, and taxonomy studies.

Key Features:

• Sourced from premium, healthy Golden Oyster mushrooms.
• Sterile and nutrient-rich for consistent mycelial growth.
• Ideal for propagation, taxonomy studies, and educational research.

Instructions for Use

Understanding the Culture Slant

A culture slant is a test tube containing nutrient agar inoculated with healthy mushroom mycelium. It is ideal for long-term preservation and propagation of fungal cultures.

Propagation from the Golden Oyster Culture Slant

1. Prepare a Sterile Work Environment:
2. • Work in a sterile area using a glove box or laminar flow hood.
   • Sterilize tools like scalpels, tweezers, and containers using flame or alcohol.
2. Inoculating Agar Plates:
3. • Carefully open the culture slant and flame the tube mouth to sterilize it.
   • Use a sterile scalpel to scrape a small section of the mycelium from the agar surface.
   • Transfer the mycelium to pre-sterilized agar plates.
   • Seal the plates and store them at 70–80°F (21–27°C) to allow mycelial colonization.
3. Preparing Grain Spawn:
4. • Once the agar plates are fully colonized, cut small sections of the colonized agar.
   • Transfer these sections to sterilized grain jars (rye, millet, or oats work well).
   • Shake the jars periodically to distribute the mycelium evenly.
4. Substrate Transfer:
5. • When the grain spawn is fully colonized, transfer it to a bulk substrate such as:
   • • Pasteurized straw
     • Hardwood sawdust
     • Supplemented substrates (bran or gypsum)
   • Golden Oyster mushrooms colonize substrates quickly due to their aggressive mycelium.
5. Fruiting Conditions:
6. • Maintain the following fruiting conditions:
   • • Temperature: 70–85°F (21–29°C) – optimal for Golden Oyster mushrooms.
     • Humidity: 85–95%.
     • Light: Indirect natural or artificial light for 12 hours per day.
   • Fruiting occurs within 7–14 days, producing beautiful clusters of golden-yellow mushrooms.

Care & Storage

• Short-Term Storage: Store the culture slant at room temperature for up to 1–2 weeks.
• Long-Term Storage: Refrigerate the culture slant at 35–40°F (2–4°C) for extended viability, up to 6–12 months.
• Sterile Handling: Always work with clean tools and surfaces to prevent contamination.

Warnings

• For Educational and Research Purposes Only: This culture slant is intended for microscopy, propagation, and educational research.
• Sterile Environment Required: Contamination can compromise the culture; always work under sterile conditions.
• Check Local Regulations: Verify local laws regarding mushroom propagation and cultivation.

Why Choose Our Golden Oyster Culture Slant?

• Premium Quality: Carefully prepared under sterile conditions to ensure purity and consistent performance.
• Fast Growth: Golden Oyster mushrooms (Pleurotus citrinopileatus) are known for their aggressive mycelial colonization and rapid fruiting.
• Vibrant Appearance: Cultivate stunning golden-yellow mushrooms ideal for educational projects and research.

About Golden Oyster Mushrooms

Golden Oyster mushrooms (Pleurotus citrinopileatus) are admired for their vivid golden-yellow caps and delicate texture. They grow rapidly on a variety of substrates and thrive in warm, humid conditions. Known for their mild, nutty flavor, these mushrooms are highly valued in culinary applications and are a rewarding species for researchers and hobbyists alike.