Lion’s Mane Culture Slant

Product Overview

The Lion’s Mane Culture Slant is a high-quality, sterile culture of the Hericium erinaceus species, revered for its unique appearance, culinary excellence, and potential medicinal benefits. This culture slant is ideal for researchers, educators, and hobbyists, providing a reliable starting point for propagation and mycological studies.

Key Features:

• Sourced from premium Lion’s Mane mushrooms.
• Prepared in a sterile environment for maximum purity and viability.
• Perfect for propagation, taxonomy studies, and educational purposes.

Instructions for Use

Understanding the Culture Slant

A culture slant is a test tube containing nutrient-rich agar inoculated with healthy mushroom mycelium. It serves as a stable medium for preserving and propagating fungal cultures.

Propagation from the Lion’s Mane Culture Slant

1. Prepare a Sterile Work Environment:
2. • Use a glove box or laminar flow hood to ensure sterile conditions.
   • Sterilize tools such as scalpels, tweezers, and containers with alcohol or flame.
2. Inoculating Agar Plates:
3. • Carefully open the culture slant and flame the mouth of the tube to sterilize it.
   • Use a sterile scalpel to scrape a small amount of mycelium from the agar surface.
   • Transfer the mycelium to pre-sterilized agar plates.
   • Seal the plates and store at 65–75°F (18–24°C) for colonization.
3. Grain Spawn Preparation:
4. • Once the agar plates are fully colonized, cut small sections of the mycelium-covered agar.
   • Transfer these agar pieces into sterilized grain jars (rye, millet, or oats).
   • Shake the jars occasionally to distribute the mycelium evenly as it colonizes the grains.
4. Substrate Transfer:
5. • Once the grain spawn is fully colonized, transfer it to a bulk substrate like hardwood sawdust or a supplemented sawdust mixture.
   • Lion’s Mane mushrooms thrive on hardwood substrates such as oak or beech.
5. Fruiting Conditions:
6. • Maintain the following conditions for fruiting:
   • • Temperature: 60–70°F (15–21°C).
     • Humidity: 85–95%.
     • Light: Indirect natural or artificial light for 12 hours per day.
   • Within 10–14 days, the substrate will produce clusters of white, cascading Lion’s Mane mushrooms.

Care & Storage

• Short-Term Storage: Store the culture slant at room temperature for up to 1–2 weeks.
• Long-Term Storage: Refrigerate the culture slant at 35–40°F (2–4°C) to maintain viability for up to 6–12 months.
• Sterile Handling: Always use clean tools and sterile environments when handling the culture slant to prevent contamination.

Warnings

• For Educational and Research Purposes Only: This culture slant is intended for microscopy, propagation, and taxonomy studies.
• Sterile Practices: Contamination will compromise the culture. Work in a sterile environment to ensure success.
• Local Regulations: Verify compliance with local laws before attempting mushroom propagation.

Why Choose Our Lion’s Mane Culture Slant?

• Premium Quality: Carefully prepared under sterile conditions to ensure viability and purity.
• Reliable Propagation: Perfect for consistent and vigorous mycelial growth.
• Unique Species: Explore the fascinating Hericium erinaceus, prized for its culinary and medicinal properties.

About Lion’s Mane Mushrooms

Lion’s Mane mushrooms (Hericium erinaceus) are renowned for their distinctive, cascading white spines, resembling a lion’s mane. Highly valued for their culinary versatility and potential cognitive benefits, Lion’s Mane mushrooms have been used in traditional medicine to support brain health and neurological function. They thrive on hardwood substrates, making them a rewarding species for research and cultivation.