Culture Slant

Shiitake Culture Slant

$54.99

Product Overview

The Shiitake Culture Slant is a high-quality, sterile culture of the Lentinula edodes species, known for its earthy flavor, nutritional benefits, and medicinal properties. This culture slant is ideal for mycology enthusiasts, researchers, and educational purposes, providing a stable and reliable foundation for propagation and study.

Key Features:

  • Sourced from premium Shiitake mushrooms.
  • Prepared in a sterile environment to ensure purity and viability.
  • Ideal for research, propagation, and educational purposes.

Instructions for Use

Understanding the Culture Slant

A culture slant is a test tube containing nutrient agar inoculated with mushroom mycelium. It is used to propagate and preserve fungal cultures over extended periods.

Propagation from the Shiitake Culture Slant

  1. Prepare a Sterile Work Environment:
    • Use a glove box or laminar flow hood to prevent contamination.
    • Sterilize tools such as scalpels, tweezers, and containers with heat or alcohol.
  2. Inoculating Agar Plates:
    • Open the culture slant carefully and sterilize the mouth of the tube with a flame.
    • Use a sterile scalpel to carefully scrape a small amount of the mycelium from the culture slant.
    • Transfer the mycelium onto pre-sterilized agar plates.
    • Seal the plates and store them at 75–80°F (24–27°C) for colonization.
  3. Grain Spawn Preparation:
    • Once the agar plates are colonized, cut small sections of the mycelium-covered agar.
    • Transfer these pieces to sterilized grain jars (rye, millet, or oats work well).
    • Allow the mycelium to colonize the grains at room temperature. Shake the jars occasionally to distribute growth.
  4. Substrate Transfer:
    • Once the grain spawn is fully colonized, transfer it to a bulk substrate such as hardwood sawdust supplemented with bran. Shiitake mushrooms thrive on hardwood-based substrates.
  5. Fruiting Conditions:
    • After colonization, expose the substrate to fruiting conditions:
      • Temperature: 55–65°F (13–18°C)
      • Humidity: 85–95%
      • Light: Indirect light for 12–14 hours per day.
    • Shiitake mushrooms will begin to pin within 7–14 days and mature into their iconic umbrella-shaped caps.

Care & Storage

  • Short-Term Storage: Keep the culture slant at room temperature for up to 1–2 weeks.
  • Long-Term Storage: Store the culture slant in a refrigerator at 35–40°F (2–4°C) to maintain viability for 6–12 months.
  • Sterile Handling: Always handle the culture slant in a sterile environment to prevent contamination.

Warnings

  • For Research and Educational Purposes Only: The culture slant is intended for microscopy, taxonomy, and learning purposes.
  • Sterile Practices: Contamination can compromise the viability of the culture. Always use sterilized tools and surfaces.
  • Legal Compliance: Ensure adherence to local regulations regarding mushroom propagation and cultivation.

Why Choose Our Shiitake Culture Slant?

  • Premium Quality: Prepared in a sterile environment to ensure purity and reliability.
  • Educational Value: Perfect for researchers and enthusiasts looking to propagate or study Shiitake mushrooms.
  • Long-Term Viability: Properly stored culture slants offer an extended shelf life for ongoing propagation.

About Shiitake Mushrooms

Shiitake mushrooms (Lentinula edodes) are revered for their rich, umami flavor and powerful medicinal properties. Traditionally used in Asian cuisine and medicine, Shiitake mushrooms are valued for their immune-boosting compounds and nutritional content. They are a favorite for both culinary enthusiasts and mycologists due to their versatility and growth on hardwood substrates.

About This Vendor

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Wild West Mushrooms

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Welcome to Wild West Mushrooms, where the bounty of nature meets innovation in the heart of Texas. Based in Austin, we’re dedicated to cultivating fresh, premium-quality mushrooms while promoting the many benefits they bring to health, culinary experiences, and sustainable living.

At Wild West Mushrooms, our mission is to connect people with the power of fungi. Whether it’s the exquisite taste of our farm-fresh mushrooms, the health-boosting potential of our triple-extract tinctures, or the joy of growing your own mushrooms, we provide everything you need to embrace the magic of mushrooms.

Fresh Mushrooms: Straight from Farm to Table

Our mushrooms are grown with care using sustainable methods that prioritize quality, flavor, and environmental stewardship. From the earthy richness of shiitake to the delicate, nutty tones of lion’s mane, our fresh mushrooms are perfect for elevating your favorite dishes. Locally grown and harvested in Austin, we ensure you’re getting the freshest product possible, bursting with nutrients and flavor.

Triple Extract Tinctures: Health and Wellness in Every Drop

Our premium line of triple-extract tinctures combines ancient wisdom with modern science. We use a meticulous extraction process that includes hot water, alcohol, and fermentation to maximize the bioavailability of essential compounds like beta-glucans and triterpenes. These tinctures are designed to support immunity, focus, energy, and overall vitality, making them an easy addition to your wellness routine.

Mushroom Growing Supplies: Cultivate Your Own Fungal Paradise

For those inspired to grow their own mushrooms, we offer high-quality growing supplies and kits. Whether you’re a beginner or a seasoned grower, our products make cultivating your own gourmet mushrooms at home a fun and rewarding experience.

Rooted in Texas, Inspired by Nature

Wild West Mushrooms is proud to be part of the vibrant Austin community, blending the independent spirit of the Lone Star State with a passion for sustainability and innovation. Our commitment to quality and authenticity reflects our deep respect for fungi’s role in health, cuisine, and the environment.

Whether you’re a mushroom enthusiast, a health-conscious consumer, or a curious beginner, Wild West Mushrooms is here to guide you on your journey. Together, let’s explore the wild wonders of mushrooms and their boundless potential.

Join us in growing a healthier, more sustainable future—one mushroom at a time.

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Must-Try for Aspiring Mushroom Cultivators!

01/29/2025

Zack Mooney

As a novice mycologist, I decided to try the Shiitake Culture Slant to experiment with growing my own mushrooms at home. I was pleasantly surprised by how user-friendly the kit was. The slant was well-prepared and provided a healthy amount of mycelium that made it easy to inoculate my substrate without any hiccups. Within just a couple of weeks, I saw the mycelium thriving! Not only is this a fantastic way to delve into mushroom cultivation, but it also feels incredibly rewarding to think about harvesting and cooking fresh Shiitakes later on. Plus, the health benefits are hard to ignore! I didn't face any contamination issues, which speaks to the quality of the slant. I will definitely be buying more and recommending it to my friends in the mycology community. Whether you're a beginner or a seasoned pro, this kit is a great addition to your mushroom-growing journey!

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