Cordyceps Militaris Liquid Culture Syringe

Unlock the Potential of Cordyceps Militaris with Our Premium Liquid Culture Syringe

Cordyceps Militaris, a potent medicinal mushroom, is prized for its wide array of potential health benefits. Cultivate your own supply of this fascinating fungus with our easy-to-use liquid culture syringe.

What is Liquid Culture?

Liquid culture is a method of propagating mushrooms using a nutrient-rich liquid solution. This syringe contains a live Cordyceps Militaris culture, ready to be injected into sterilized grain or other suitable substrates to start your mushroom growing journey.

Potential Benefits of Cordyceps Militaris:

• May boost energy and stamina
• Supports immune system function
• Could enhance athletic performance
• Traditional use for respiratory health
• Potential antioxidant properties

Using Our Cordyceps Militaris Liquid Culture Syringe

1. Prepare a sterilized substrate (grain jars are common).
2. Under sterile conditions, inject a small amount of the liquid culture into your substrate.
3. Incubate in a suitable environment (refer to our Cordyceps growing guide [link to guide]).

Storage: Store your liquid culture syringe in the refrigerator until ready to use.

Get Growing Today!

Experience the power of homegrown medicinal mushrooms. Order your Cordyceps Militaris liquid culture syringe and start your cultivation journey!

Growing Cordyceps mushrooms using a liquid culture syringe involves several options: agar expansion, liquid culture expansion, and substrate inoculation. Here are detailed instructions for each stage:

Materials Needed:

• 10cc Cordyceps liquid culture syringe
• Agar plates (pre-prepared or homemade)
• Sterilized grain jars (e.g., brown rice, millet, or another suitable grain)
• Sterilized substrate (e.g., a mix of brown rice flour, vermiculite, and water)
• Sterile gloves
• Alcohol wipes
• Flame source (e.g., lighter or alcohol lamp)
• Clean working area (preferably a still air box or a laminar flow hood)
• Pressure cooker or autoclave (for sterilizing substrate)
• Sterile water and containers for liquid culture
• Aluminum foil
• Masking tape and labels

Step 1: Agar Expansion

1. • Prepare Agar Plates: If using homemade agar plates, prepare the agar medium (e.g., potato dextrose agar or malt extract agar) and pour into sterile petri dishes. Allow the agar to solidify.
   • Sterilize Tools: Clean your working area thoroughly. Sterilize your scalpel and inoculating loop by heating until red-hot. Allow them to cool.
2. • Set Up a Clean Work Area: Use a still air box or laminar flow hood. Wear sterile gloves and disinfect them with alcohol.
   • Inoculate the Agar: Flame sterilize the needle of the syringe until it glows red, then let it cool. Inject a small amount (1-2 drops) of the Cordyceps liquid culture onto the agar plate. Use the sterile inoculating loop to spread the culture evenly across the agar surface.
   • Seal the Plates: Close the petri dishes and seal the edges with parafilm or plastic wrap to prevent contamination.
3. • Store the Plates: Place the inoculated plates in a dark, warm area with a stable temperature of 68-77°F (20-25°C).
   • Monitor Growth: Check the plates periodically for signs of mycelial growth, which should appear within a week. Fully colonized agar will be covered in white mycelium.

Step 2: Liquid Culture Expansion

1. • Prepare Liquid Culture Medium: Mix 4% honey or light malt extract with water and pour into sterilizable containers (e.g., jars with self-healing injection ports). Sterilize the medium in a pressure cooker for 30 minutes at 15 psi. Allow to cool.
   • Sterilize Tools: Sterilize a syringe and needle, as well as any other equipment you will use.
2. • Set Up a Clean Work Area: Use a still air box or laminar flow hood. Wear sterile gloves and disinfect them with alcohol.
   • Inoculate the Liquid Culture: Flame sterilize the needle, let it cool, and inject 1-2cc of the Cordyceps liquid culture into the sterilized liquid medium through the injection port. Seal the port with sterile tape.
   • Mix Thoroughly: Shake the jar gently to distribute the culture throughout the liquid.
3. • Store the Jars: Place the inoculated liquid culture jars in a dark, warm area at 68-77°F (20-25°C).
   • Monitor Growth: Agitate the jars daily to ensure even distribution of the mycelium. The liquid culture should show signs of mycelial growth within a week and be ready for use within 2-3 weeks.

Step 3: Substrate Inoculation

1. • Prepare the Substrate: Mix your substrate ingredients (e.g., 2 parts brown rice flour, 1 part vermiculite, 1 part water) and fill your jars about 2/3 full. Sterilize the substrate in a pressure cooker at 15 psi for 90 minutes. Allow to cool.
   • Sterilize Tools: Clean your working area thoroughly. Sterilize a syringe needle and any other tools you will use.
2. • Set Up a Clean Work Area: Use a still air box or laminar flow hood. Wear sterile gloves and disinfect them with alcohol.
   • Inoculate the Jars: Flame sterilize the needle, let it cool, and inject 1-2cc of the Cordyceps liquid culture from the expanded liquid culture jar into each sterilized substrate jar. Distribute the culture evenly.
   • Seal the Jars: Cover the inoculation site with sterile micropore tape to prevent contamination.
3. • Store the Jars: Place the inoculated jars in a dark, warm area at 68-77°F (20-25°C). Maintain humidity levels around 70-75%.
   • Monitor Growth: Check the jars periodically for signs of colonization. The substrate should be fully colonized within a few weeks, appearing white and fuzzy.