King Oyster Culture Slant

Product Overview

The King Oyster Culture Slant is a premium, sterile culture of the Pleurotus eryngii species, known for its thick, meaty stems, mild flavor, and excellent culinary versatility. This culture slant is perfect for researchers, educators, and hobbyists seeking to propagate and study this unique and robust mushroom species.

Key Features:

• Sourced from high-quality, mature King Oyster mushrooms.
• Sterile and nutrient-rich to ensure reliable mycelial growth.
• Ideal for propagation, taxonomy research, and educational purposes.

Instructions for Use

Understanding the Culture Slant

A culture slant is a test tube containing nutrient-rich agar inoculated with healthy mycelium. It is designed for long-term storage and mycelial propagation.

Propagation from the King Oyster Culture Slant

1. Prepare a Sterile Work Environment:
2. • Work in a clean, sterile area using a glove box or laminar flow hood.
   • Sterilize tools such as scalpels, tweezers, and jars with alcohol or flame.
2. Inoculating Agar Plates:
3. • Open the culture slant carefully and flame the mouth of the tube to sterilize it.
   • Use a sterile scalpel to scrape a small piece of mycelium from the agar.
   • Transfer the mycelium to pre-sterilized agar plates and seal them.
   • Incubate at 65–75°F (18–24°C) to allow mycelial colonization.
3. Preparing Grain Spawn:
4. • Once the agar plates are fully colonized, cut small sections of the mycelium-covered agar.
   • Transfer the agar pieces to sterilized grain jars (rye, oats, or millet).
   • Shake the jars periodically to distribute the mycelium evenly as it colonizes the grains.
4. Substrate Transfer:
5. • Transfer the fully colonized grain spawn to a bulk substrate, such as:
   • • Hardwood sawdust
     • Supplemented sawdust blocks (bran or gypsum)
     • Straw
   • King Oyster mushrooms grow best on hardwood-based substrates.
5. Fruiting Conditions:
6. • Introduce the following fruiting conditions:
   • • Temperature: 60–70°F (15–21°C).
     • Humidity: 85–95%.
     • Light: Indirect light for 12 hours per day.
   • Fruiting typically occurs within 10–14 days, producing large clusters of thick, meaty King Oyster mushrooms.

Care & Storage

• Short-Term Storage: Store the culture slant at room temperature for up to 1–2 weeks.
• Long-Term Storage: Refrigerate the culture slant at 35–40°F (2–4°C) to extend viability for up to 6–12 months.
• Sterile Handling: Always handle the culture in a sterile environment to prevent contamination.

Warnings

• For Educational and Research Purposes Only: The King Oyster culture slant is intended for microscopy, taxonomy, and propagation experiments.
• Sterile Techniques Required: Contamination will compromise the culture; work in a clean, sterile environment.
• Local Regulations: Ensure compliance with local laws regarding mushroom cultivation and propagation.

Why Choose Our King Oyster Culture Slant?

• Premium Quality: Carefully prepared under sterile conditions for purity and consistency.
• Reliable Propagation: Perfect for mycelial expansion and bulk substrate cultivation.
• Unique Species: Explore the Pleurotus eryngii, one of the most robust and rewarding gourmet mushrooms.

About King Oyster Mushrooms

King Oyster mushrooms (Pleurotus eryngii) are prized for their thick, cylindrical stems and mild, umami flavor, making them a favorite in culinary applications. They are highly versatile, growing well on hardwood substrates and thriving in cool, humid conditions. Known for their meaty texture, King Oyster mushrooms are a rewarding species for propagation and study.